microRNAs (miR 9, 124, 155 and 224) transdifferentiate mouse macrophages to neurons

Development is an irreversible process of differentiating the undifferentiated cells to functional cells. Brain development involves generation with varied phenotype and functions, which limited during adulthood, stress, damage/degeneration. Cellular reprogramming makes differentiation reversible somatic/stem alternative fate with/without stem Exogenously expressed transcription factors or small molecule inhibitors have driven stem/somatic neurons providing approach for pre-clinical/clinical testing therapeutics. Here in, we report a novel microRNA (miR)- induced trans-differentiation macrophages (CD11b high) neuronal (iNCs) (neuronal markershigh- Nestin, Nurr1, Map2, NSE, Tubb3 Mash1) without exogenous use factors. miR 9, 124, 155 224 successfully transdifferentiated transient cell-like phenotype. We trans efficacy 18% 21% 124 155. in silico(String 10.0, gator, mESAdb, TargetScan 7.0) experimental analysis indicate that alteration pluripotencygenes like Oct4, Sox2, Klf4, Nanog pluripotency miR, 302. iNCs also shifted G0 phase indicating manipulation cell cycle by these miRs. Further, CD133+ intermediate obtained current protocol could be differentiated using The syanpsin+ were functionally active displayed intracellular Ca+2 evoke on activation. miRs transdifferentiate bone marrow-derived peripheral blood mononuclear employed direct vivo teratoma formation transplantation clinical studies.